Dr. Hermann E. Gaub, head of the Biophysics and Molecular Materials Group in the Physics Department at the Ludwig-Maximilians-University (LMU) of Munich, together with Elias Puchner and colleagues from the university's Center for Nanoscience and the Center for Integrated Protein Science Munich, combined the precision of atomic force microsccopy with the selectivity of DNA interaction to create freely programmable nanopatterns of DNA-oligomers on a surface and in aqueous environment.

What the LMU researchers did was create a DNA scaffold by picking biotin bearing DNA oligomers with an AFM tip and depositing them, one by one, in a desired pattern on a surface, basically creating a pattern of attachment points for fluorescent semiconductor nanoparticles conjugated with streptavidin. The small bacterial protein streptavidin is commonly used for the detection of various biomolecules and it binds with high affinity to the vitamin biotin. The strong streptavidin-biotin bond can be used to attach various biomolecules to one another or onto a solid support.

When the sample with the DNA scaffold is incubated with a solution of fluorescent nanoparticles, a rapid self-assembly process of these particles on the predefined scaffold takes place.