The goal for this Center is to investigate and manipulate the complex cell signaling/regulatory network circuitries in a time-resolved dynamic manner in order to not only enhance our understanding of individual pathways but also provide vital information on their co-dependence and interactivities for the potential of controlling the cell phenotype. Our unique approach employs a systemic view of controlling networks that has the ability to i) perturb the cellular network circuitry, ii) conduct real time monitoring of the key signaling elements, iii) interpret and analyze the system's response, and iv) use a global system control algorithm to dissect and analyze the cellular network circuitry. This approach addresses the NIH's vision on NDC that “ decisions on the biological systems…guided by the principle that the precise control required to manipulate cellular components will depend upon understanding and using engineering principles”. Our objective is not only to extend our understandings of local interactions of cellular components but also globally control cellular functions through a network of circuitries.